Ultra High Performance Liquid Chromatography (UHPLC)

• Shorter and smaller inside-diameter columns used

•  2 µm stationary phase

• System frequently runs at pressures in excess of 600 bar

• Requires more-sophisticated instrumentation due to very high pressure that the system needs to withstand

• Use of UHPLC improves productivity and throughput, also reduces solvent consumption

• UHPLC provides significant advantages in terms of speed, peak resolution, sensitivity and peak capacity.

Source: http://www.dddmag.com/articles/2010/12/liquid-chromatography-evolution